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KMID : 0380020110260040305
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Korean Journal of Biotechnology and Bioengineering
2011 Volume.26 No. 4 p.305 ~ p.310
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The Early Detection of the Spore Using Sonication and Fluorescent Dye in the Field
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Ha Yeon-Chul
Choi Ki-Bong
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Abstract
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This study was carried out to establish the optimum condition of cell disruption with a sonicator for the detection of the spore, Bacillus anthracis △-sterne for the purpose of developing automatic fluorometer. The efficiency of sonication on the
△-sterne spore disruption was very weak. The △-sterne spore with zirconia bead showed greater disruption than the
△-sterne spore alone when sonificated. The volumn of the zirconia bead added in the spore solution has little effect on the disruption efficiency. The detection limit of the △-sterne spore with zirconia bead and the △-sterne spore alone was 106 CFU/mL and CFU/mL and 5x107 CFU/mL respectively, when sample was sonicated for 20 seconds with a sonicator probe of 13 mm diameter.
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KEYWORD
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sonication, Fluorescent, Spore, Zirconia
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